The Aurora SARS-CoV-2 PLpro assay kit is a homogeneous assay for screening PLpro Inhibitors. The assay is fast and convenient, and requires just two steps. In the first step, the PLpro enzyme is preincubated with the compound for 30 minutes. The reaction is initiated by adding substrate solution at the second step. Fluorescent intensity is measured with a fluorescent plate reader at the excitation wavelengths of 340-360 nm and emission wavelengths of 460-480 nm. Materials Supplied: 2X Protease Assay Buffer(20ml), 0.5 M DTT(200ul), 5 mM Substrate (10ul), Recombinant SARS-CoV-2 PLpro (5ug), 1x Black low binding 96 well plate Materials Needed But Not Supplied: A microplate reader capable of measuring fluorescence at excitation wavelengths of 340-360 nm and emission wavelengths of 460-480 nm.